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PrimeQ® Real-Time PCR System
Click here to request a web demo (via Webex) of PrimeQ's software package, Quansoft!
PrimeQ® has been designed with the advantage of having an open architecture and chemistry format that allows the end user full flexibility of the methods and research they wish to pursue.
Quansoft® software and reporting
Accompanying PrimeQ® is our easy-to-use, flexible software Quansoft®, which gives you the freedom to see your raw data, choose your disposables and choose your own chemistries!
Here's just some of the things you can finally do with Quansoft®:
-ability to create, modify and save files using Quansoft® while a program is running on the unit
-ability to run without initially defining your plate layout and analysis criteria - just pop a prepared plate in & run a program
-ability to export unprocessed, raw data
-install Quansoft® on multiple PCs without paying a license fee (only a simple registration is required)
-ability to change from first order derivative to fit points in results analysis
Here's just some of the things you can finally do with Quansoft®:
-ability to create, modify and save files using Quansoft® while a program is running on the unit
-ability to run without initially defining your plate layout and analysis criteria - just pop a prepared plate in & run a program
-ability to export unprocessed, raw data
-install Quansoft® on multiple PCs without paying a license fee (only a simple registration is required)
-ability to change from first order derivative to fit points in results analysis
Features
- Solid state white light source and PMT (photomultiplier tube) detector gives an impressive excitation range of 470nm to 650nm and a detection range of 500nm to 710nm.
- Multiplex - multiple wavelengths are detectable per sample using up to four paired excitation and emission filters housed in individual cartridge systems.
-Wide dynamic range - up to at least 9 orders of magnitude from starting copy number and high sensitivity, detecting down to 1nM fluorescein and single copy templates, depending upon the assay.
- Multiplex - multiple wavelengths are detectable per sample using up to four paired excitation and emission filters housed in individual cartridge systems.
-Wide dynamic range - up to at least 9 orders of magnitude from starting copy number and high sensitivity, detecting down to 1nM fluorescein and single copy templates, depending upon the assay.
Heated Lid
The flexible optical heated lid system is designed to minimize loss of sample and prevent sample condensation. Temperature controlled, it allows further method optimization where pre-incubation stages may be required (e.g. in reverse transcription techniques) or where changes in the heated lid characteristics are needed during the different stages of a program run.
Technical Specifications
| Parameter | PrimeQ® |
|---|---|
| Block format | 96x0.2ml |
| Block specification | 8 x Peltier block employing quad-circuit technology to enhance performance |
| Block uniformity at 50°:C | Better than ±:0.3°C |
| Maximum ramp rate | Up to 2.2°C |
| Temperature range | 4 to 98°C |
| Sample volume | 15 to 50ul |
| Heated lid | Adjustable between 100 and 115°C in 1°C increments, or off |
| Maximum sample evaporation | Less than 15 volume loss from any well using the plate sealing recommended by Techne |
| PC connectivity | USB, OS Win XP SP3 or higher and Win 7 (one unit per PC) |
| Plate format | Low profile 96-well plate (skirted or non-skirted) |
| Excitation source | Solid state white light source |
| Detector | Photon counting photomultiplier tube |
| Multiplex dye detection | Up to 4 dyes per reaction tube |
| User selec ted filters | Maximum of 4 paired excitationemission filter cartridge systems suitable wth currently used dyes* |
| Fluorescence excitation range | 470-650nm (standard filters are centered at 485, 530, 580 and 640nm) |
| Fluorescence detection range | 500-710nm (standard filters are centered at 520, 560, 615 and 685nm) |
| Dynamic range | At least 9 orders of magnitude of target DNA concentration |
| Sensitivity (detection threshold) | 1nM fluorescein in a 20ul sample |
| Resolution | 1.5 fold difference in target DNA concentration |
| Weight | 55 lbs |
| Dimensions, WxHxD, in. | 16 x 20 x 21 |
| Input power | 715W |
| Power supply | 100-230V, 5060Hz |
*custom filter cartridge systems available upon request
Ordering Information
| Part No. | Description |
|---|---|
| PrimeQ | PrimeQ Real-Time PCR System |
| FC01 | FAM multiplex, 460nm excitation wavelength, 500nm emission wavelength |
| FC02 | Green, FAM|SYBR®, 485nm excitation wavelength, 520nm emission wavelength |
| FC03 | Yellow, HEX, 530nm excitation wavelength, 560nm emission wavelength |
| FC04 | Red, ROX, 580nm excitation wavelength, 615nm emission wavelength |
| FC05 | Blue, Cy5, 640nm excitation wavelength, 685nm emission wavelength |
*Please note FC01 is not included with the unit.
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