Endurance TC412 Thermal cycler

Specifications
Blocks Formats
0.5ml capacity	60
0.2ml capacity	96
384-well block	Yes
In-situ block	Yes
User interchangeable	Yes
Block Temperature Specifications
Maximum ramp rate*	2.6°C/sec
Block uniformity at 50°C*	<±0.3°C
Temperature range	4°C to 99°C
Temperature set point precision	0.1°C
Heated Lid
Selectable heated lid temperature	100°C - 115°C
Heated lid enable/disable	Yes
Over temperature cut out	Yes
Regulated lid pressure	Yes
Heated lid only comes on if block is set above 35°C
Programming
Number of programs #	80
Password protection	Yes
Programming	4-line alphaN
Graphical display	No
Incremental/decremental temperature	Yes
Incremental/decremental hold time	Yes
Maximum hold time	18 hrs
Minimum hold time	1 sec
Variable programmable ramp rate	0.1°C/sec steps
Run end time calculation	Yes
Pause and stop facility	Yes
End of program alarm (enable/disable)	Yes
Auto restart on power failure	Yes
Miscellaneous
Serial port	RS232
Dual Voltage (120/230V)	Yes
Temperature sensor	Thermistor
Peltierelements/block	8
Dimensions L x W x H (in inches)	16.5 x 8.6 x 10.2
Footprint L x W (in inches)	142 in²
Connection to PC control program	Yes
Free software upgrades from website	Yes
Power consumption	620 Watts
Frequency	50 - 60 Hz
Net weight	11kg (total)
Warranty	4 years or 80,000 cycles-parts and labor
* Given are typical values of a standard 0.2ml block, in an ambient temperature of 20°C. # Actual numbers are dependent on complexity of the programs.

Ordering Information
*Catalog Number*	*Description*	*Voltage*
FTC41H2D	TC-412 96 x 0.2mL microtubes or 96-well plate (half or no skirt)	120/230
FTC41S5D	TC-412 60 x 0.5mL microtubes	120/230
FTC4384D**	TC-412 384-well microtitre plate	120/230
FTC41FLD**	TC-412 Flat Plate for in-situ	120/230
FTC4QH2D	TCQ-412 96 X 0.2mL microtubes or 96 well plates (includes 4 Thermal cyclers and all accessories required to connect units to each other)	120/230
FTC4Q38D**	TCQ-412 384-well microtitre plate (includes 4 Thermal cyclers and all accessories required to connect units to each other)	120/230
	*Accessories*
FTC41BHD	Block for 96 x 0.2mL microtubes or 96-well plate (Half or no skirt)	Block
FTC41B5D	Block 60 x 0.5mL microtubes	Block
FTC4B384**	Block for 384-well microtitre plate	Block
FTC41BID**	Flate plate block for in-situ	Block
7022740	Aluminum PCR sealing Film, non sterile - 100 pack - $71.40 (Compare our price to others)
7022741	Aluminum PCR sealing Film, Sterile - 50 pack - $45.82 (Compare our price to others)
7022556	Gensoft software for 1-5 units free from website Click here to download	Software
7022563	Gensoft software for 6-32 units. Please contact Techne®	Software
Thermal Cycler tubes and accessories
The items below are required to connect multiple TC412's to your PC to create a thermal cycler network. (Note: These are included in the TCQ-412 system above.)
7022558	Terminator	120 volt
7022557	Terminator	240 volt
7022559	One cycler cable extension kit	cable kit
7022560	Two cycler cable kit	cable kit
7022690	Four cycler cable kit	cable kit
7022562	Ten cycler cable kit	cable kit
** Available by special order only

Block temperatures
To provide thermal cyclers with the high performance and specificity required for the protocols of today, heated block design and program versatility are important features.

Incremental/decremental temperatures
Touchdown DNA protocols are where a higher temperature is required for the initial cycles than later cycles. This ensures that only highly specific product is created (amplified) during the critical initial steps. A decreased, less stringent temperature is then used by gradually decreasing the annealing temperature. The effect is a higher yield and more specific product. Therefore, to eliminate the need for programming lengthy error prone protocols, choose a thermal cycler which can automatically incorporate an incremental decrease/increase in temperature.

Incremental/decremental time
Long template DNA is the amplification of large fragments of DNA (e.g. 40kb lambda, 27kb Genomic). To ensure that the extension is fully complete after each cycle, the extension time is increased with each cycle to compensate for the increased product quantity and decreasing concentrations of reagents. As above with temperature, choose a thermal cycler which can automatically increase/decrease hold time at a given temperature.

Peltier technology
The peltier effect is used in many applications for the reliable cooling of sensitive high-temperature components without the need for refrigerants or compressors. Otherwise known as thermoelectric coolers, peltiers are a special type of semiconductor that function as a solid state heat pump. By applying a low voltage DC power source to one side, heat will be moved in the direction of the current (+ to - ). The heat is thus pumped from one side of the module to the other, so that one face will be cold while the opposite face will be heated. As this effect is reversible, peltier technology is the chosen method for heating and cooling the block in the best thermal cyclers on the market today.